Strain Name
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NOD.CB17-PrkdcscidIl2rgtm1BcgenThpotm1(THPO)Bcgen/Bcgen
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Common Name
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huHSC-B-NDG hTHPO mice
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Background
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B-NDG
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Catalog number
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112480
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Aliases
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MGDF, MKCSF, ML, MPLLG, THCYT1, TPO
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B-NDG hTHPO mice are well suited for human immune system engraftment
Human CD34+ HSCs (5E5) were intravenous engrafted into wild-type B-NDG mice and homozygous B-NDG hTHPO mice (female, 6 week-old, n=20). B-NDG mice were treated with 2Gy-irradiation. B-NDG hTHPO mice were not irradiated. Flow cytometric analysis of peripheral blood lymphocyte from the two mice after engraftment with human CD34+ HSCs were shown in the figure above. Results showed that the survival rate of B-NDG hTHPO mice was higher than that of B-NDG mice. The percentages of human CD45+ leukocytes, T cells, B cells, NK cells, myeloid cells, monocytes, macrophages and neutrophils in B-NDG hTHPO mice were similar to those in B-NDG mice, demonstrating that the humanized mouse model engrafted with human CD34+ HSCs was successfully constructed. Values are expressed as mean ± SEM. HSCs: hematopoietic stem cells.